BU Research Blog

 I had the pleasure of spending the last three weeks in the Department of Life Sciences (University of Roehampton), working with Dr Giulia Corona and Dr Volker Behrends. We successfully validated an ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS) method to analyse lipid peroxidation products (breakdown products of fats) in human urine samples.

Our ultimate goal is to identify the time-course of oxidative stress and subsequent breakdown of lipids in neonates, by analysing urinary lipid peroxides, to facilitate evidence-based approaches to antioxidant support in preterm neonates. My supervisors Dr Simon Dyall and Prof Minesh Khashu and I are currently developing a pilot study in cooperation with Poole Hospital NHS Foundation Trust to test the feasibility of measuring these products in urine samples from preterm neonates. However, before moving on to preterm samples, the method had to be tested and validated, using adult urine samples.

The project was made possible by an HSS Seed Corn fund obtained by Dr Simon Dyall, the PGR fund of Isabell Nessel, the collaboration with Dr Giulia Corona and Dr Volker Behrends at the Department of Life Sciences (University of Roehampton) and collaboration with Dr Thierry Durand and his group from the Institute des Biomolecules Max Mousseroux, (Montpellier, France) who generously provided the standards used in this analysis. I am grateful that I had this opportunity to further develop my research skills and to learn a new state-of-the-art technique and would like to thank all involved for making this possible!

If you would like to learn more about our research please feel free to contact me at inessel@bournemouth.ac.uk

Isabell